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A3(人T淋巴細胞白血病細胞)

CBP60703

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I. General information 
Synonyms: A3
Background: The A3 subclone was derived from a Jurkat cell line obtained from the laboratory of Gerald Crabtree at Stanford University.
Species: Homo sapiens, human
Disease: acute T cell leukemia
Gender: male
Morphology: lymphoblast
Growth Mode: suspension
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 17,18
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Culture Medium:

RPMI-1640 +10%FBS

A3完全培養(yǎng)基,# CBP60703M
We strongly suggest to purchase the complete medium from us.

Cryopreservation medium: 90%FBS+10%DMSO
Comments: The Jurkat cells were treated with Fas Antibody and isolated by limiting dilution to obtain a cell line that had a low spontaneous rate of resistance to Fas-medicated apoptosis.The resulting wild-type A3 subclone is very sensitive to Fas-mediated apoptosis.Wild-type A3 cells were made neomycin resistant and treated with three cycles of exposure to the frameshifting mutagen ICR-191 to isolate clones harboring recessive mutations that were resistant to killing by Fas antibody.
ICR-191 treated clones were serially diluted in 96-well plates in the presence of Fas Antibody for 3 to 5 weeks.Two of these ICR-191 treated clones have been deposited at the ATCC. They are I 9.2 (ATCC CRL-2571), a clone with a mutation in the cysteine protease caspase-8/FLICE and I 2.1 (ATCC CRL-2572), a clone with a mutation in the adaptor FADD.
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